Sensing Application — Aptasensor based on gold nanostructure-decorated 2D Cu metal–organic framework nanosheets for highly sensitive and specific electrochemical lipopolysaccharide detection

6 measurement groups · 25 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

Apt/Au/Cu-THQ/GCE · Electrode

Human serum samples from sepsis cases measured by Apt/Au/Cu-THQ/GCE and compared with WB-80 microbial dynamic monitoring system.

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Clinical serum sample 1 LPS by biosensorMarked as a best value within this paper	1.33 +/- 0.29 pg/mL; WB-80 <5.00 pg/mL	1.33 pg/mL	+/- 0.29 pg/mL; n = 3	Table Exact Reported	9 · Serum sample detection · Table 2
Clinical serum sample 2 LPS by biosensor	8.47 +/- 1.07 pg/mL; WB-80 8.08 pg/mL	8.47 pg/mL	+/- 1.07 pg/mL; n = 3	Table Exact Reported	9 · Serum sample detection · Table 2
Clinical serum sample 3 LPS by biosensor	15.15 +/- 1.35 pg/mL; WB-80 15.43 pg/mL	15.15 pg/mL	+/- 1.35 pg/mL; n = 3	Table Exact Reported	9 · Serum sample detection · Table 2
WB-80 standard method LOD	LOD = 5 pg/mL	5 pg/mL	—	Text Exact Reported	9 · Serum sample detection · Table 2

Apt/Au/Cu-THQ/GCE · Electrode

DPV response to LPS concentrations from 0 to 1e-10 g/mL; calibration of peak current versus logarithm of LPS concentration.

Temperature: 310
Geometry: Apt/Au/Cu-THQ/GCE working electrode
Context: final aptasensor
Measurement source: 8 · LPS detection performance of Apt/Au/Cu-THQ/GCE · Figure 4A,B

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
LPS calibration intercept	Ip = 37 lgcLPS + 313	—	—	Text Exact Reported	8 · LPS detection performance of Apt/Au/Cu-THQ/GCE · Figure 4B
LPS calibration R2Marked as a best value within this paper	R2 = 0.9992	—	—	Text Exact Reported	8 · LPS detection performance of Apt/Au/Cu-THQ/GCE · Figure 4B
LPS calibration slope	Ip = 37 lgcLPS + 313	—	—	Text Exact Reported	8 · LPS detection performance of Apt/Au/Cu-THQ/GCE · Figure 4B
Langmuir dissociation constant for LPS bindingMarked as a best value within this paper	KD = 3.25 fg/mL	0.00325 pg/mL	—	Text Exact Reported	8 · LPS detection performance of Apt/Au/Cu-THQ/GCE · Figure S13
LPS linear range upper boundMarked as a best value within this paper	100 pg/mL	100 pg/mL	—	Text Exact Reported	8 · LPS detection performance of Apt/Au/Cu-THQ/GCE · Figure 4A,B
LPS linear range lower boundMarked as a best value within this paper	1 fg/mL	0.001 pg/mL	—	Text Exact Reported	8 · LPS detection performance of Apt/Au/Cu-THQ/GCE · Figure 4A,B
LPS limit of detectionMarked as a best value within this paper	0.15 fg/mL (S/N = 3)	0.00015 pg/mL	S/N = 3	Text Exact Reported	8 · LPS detection performance of Apt/Au/Cu-THQ/GCE · Figure 4A,B; Table S1

Apt/Au/Cu-THQ/GCE · Electrode

Five biosensors measured for 1 pg/mL LPS reproducibility; biosensor stored at 4 C for 15 days and remeasured for 1 pg/mL LPS.

Temperature: 277
Geometry: Apt/Au/Cu-THQ/GCE working electrode
Context: final aptasensor
Measurement source: 8 · LPS detection performance of Apt/Au/Cu-THQ/GCE · Tables S2-S3

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Reproducibility sample 1 DPV peak current	130 uA	—	—	SI Table Exact Reported	4 · Supplementary Materials · Table S2
Reproducibility sample 5 DPV peak current	142 uA	—	—	SI Table Exact Reported	4 · Supplementary Materials · Table S2
Reproducibility RSD for 1 pg/mL LPSMarked as a best value within this paper	3.98% (n = 5)	—	n = 5	Text Exact Reported	8 · LPS detection performance of Apt/Au/Cu-THQ/GCE · Table S2
SI Table S2 reproducibility RSDMarked as a best value within this paper	4.0% for five DPV peak-current measurements	—	—	SI Table Exact Reported	4 · Supplementary Materials · Table S2
Storage stability day 15 average DPV current	357 +/- 3 uA	—	—	SI Table Exact Reported	4 · Supplementary Materials · Table S3
Storage stability day 1 average DPV current	393 +/- 1 uA	—	—	SI Table Exact Reported	4 · Supplementary Materials · Table S3
Storage stability current decrease after 15 daysMarked as a best value within this paper	9 +/- 1%	—	—	SI Table Exact Reported	4 · Supplementary Materials · Table S3
Current retained after 15 days at 4 CMarked as a best value within this paper	91% of initial current (n = 3)	—	n = 3	Text Exact Reported	8 · LPS detection performance of Apt/Au/Cu-THQ/GCE · Table S3

Apt/Au/Cu-THQ/GCE · Electrode

DPV responses measured with Glu, IgGs, HSA, AST, ALT and LPS, each at 0.1 pg/mL.

Geometry: Apt/Au/Cu-THQ/GCE working electrode
Context: final aptasensor
Measurement source: 8 · LPS detection performance of Apt/Au/Cu-THQ/GCE · Figure 4C,D

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
HSA interferent delta current	about 26 uA from Figure 4D	—	visual estimate from bar chart	Figure Axis Approximate	8 · LPS detection performance of Apt/Au/Cu-THQ/GCE · Figure 4D
LPS selectivity response delta currentMarked as a best value within this paper	about 98 uA from Figure 4D	—	visual estimate from bar chart	Figure Axis Approximate	8 · LPS detection performance of Apt/Au/Cu-THQ/GCE · Figure 4D

Apt/Au/Cu-THQ/GCE · Electrode

DPV peak currents with and without 0.1 pg/mL LPS compared in 1x PBS buffer and serum.

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Buffer and serum matrix response comparisonMarked as a best value within this paper	currents recorded for LPS in buffer and serum were nearly identical	—	—	Text Qualitative	9 · Serum sample detection · Figure S14

Apt/Au/Cu-THQ/GCE · Electrode

LPS measured in 50-fold diluted human serum spiked at 1.00e-13, 1.00e-12 and 1.00e-11 g/mL.

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Spiked serum detected LPS at 1.00e-11 g/mL added	1.08e-11 g/mL; recovery 108%; RSD 5.3%	10.8 pg/mL	RSD 5.3%; n = 3	Table Exact Reported	9 · Serum sample detection · Table 1
Spiked serum detected LPS at 1.00e-12 g/mL added	0.98e-12 g/mL; recovery 98%; RSD 3.5%	0.98 pg/mL	RSD 3.5%; n = 3	Table Exact Reported	9 · Serum sample detection · Table 1
Spiked serum detected LPS at 1.00e-13 g/mL added	1.05e-13 g/mL; recovery 105%; RSD 8.8%	0.105 pg/mL	RSD 8.8%; n = 3	Table Exact Reported	9 · Serum sample detection · Table 1