Electrochemistry Application — Laccase-inspired bi-amino acid MOFs with high substrate affinity: Catalytic deposition induced “signal-down” electrochemical response towards PD-L1

8 measurement groups · 26 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

storage stability catalytic activity assay

Cu-F/Hs powder/nanoparticles · Powder

Relative catalytic activity monitored over 20 days.

Context: pristine Cu-F/Hs catalytic material
Measurement source: 8 · 3.5 · Fig. 6D

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Relative catalytic activity after 20 days	more than 85%	—	—	Text Approximate	8 · 3.5 · Fig. 6D

cyclic voltammetry

Voltammetry

PD-L1 sandwich sensor on gold electrode · Electrode

CV curves of step-by-step sensor construction and signal amplification.

Geometry: three-electrode gold-electrode sensor
Context: composite sensor electrode
Measurement source: 6 · 3.2 · Fig. 3B

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
CV current signal variation after PD-L1	5.07 uA	—	—	Text Exact Reported	6 · 3.2 · Fig. 3B
CV current signal after PDA amplification	9.07 uA	—	—	Text Exact Reported	6 · 3.2 · Fig. 3B

EIS impedance-change optimisation

Impedance spectroscopy

Cu-F/Hs with ODA in DA polymerisation mixture · Unknown

DA polymerisation impedance changes measured with/without ODA and Cu-F/Hs, and with BDA, DACH, HDA, or ODA.

Geometry: electrode impedance assay
Context: Cu-F/Hs catalytic reaction mixture and electrode product
Measurement source: 5 · 3.3 · Fig. 4B,D

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Cu-F/Hs plus DA impedance change	about 700 ohm	—	error bar shown	Figure Axis Approximate	5 · 3.3 · Fig. 4B
Cu-F/Hs plus DA plus ODA impedance changeMarked as a best value within this paper	about 1260 ohm	—	error bar shown	Figure Axis Approximate	5 · 3.3 · Fig. 4B
DA-only impedance change	about 240 ohm	—	error bar shown	Figure Axis Approximate	5 · 3.3 · Fig. 4B
DA plus ODA impedance change	about 360 ohm	—	error bar shown	Figure Axis Approximate	5 · 3.3 · Fig. 4B
BDA-promoted DA polymerisation impedance change	about 930 ohm	—	error bar shown	Figure Axis Approximate	5 · 3.3 · Fig. 4D
DACH-promoted DA polymerisation impedance change	about 880 ohm	—	error bar shown	Figure Axis Approximate	5 · 3.3 · Fig. 4D
HDA-promoted DA polymerisation impedance change	about 1000 ohm	—	error bar shown	Figure Axis Approximate	5 · 3.3 · Fig. 4D
ODA-promoted DA polymerisation impedance changeMarked as a best value within this paper	about 1290 ohm	—	error bar shown	Figure Axis Approximate	7 · 3.3 · Fig. 4D

electrochemical impedance spectroscopy

Impedance spectroscopy

PD-L1 sandwich sensor on gold electrode · Electrode

Stepwise electrode construction measured in 5 mM [Fe(CN)6]3-/4- over 0.1-100 kHz and fitted with Randles circuit using CPE.

Geometry: three-electrode cell; gold working electrode, saturated calomel reference, platinum counter
Context: composite sensor electrode
Measurement source: 4 · 2.3; 3.2 · Fig. 3A

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Rct signal change after PD-L1 capture	610 ohm	—	—	Text Exact Reported	6 · 3.2 · Fig. 3A
Rct change after PDA catalytic deposition	1130 ohm	—	—	Text Exact Reported	6 · 3.2 · Fig. 3A

Michaelis-Menten kinetic assay by UV-vis

UV-visible and optical absorption spectroscopy

Cu-F/Hs powder/nanoparticles · Powder

DA substrate oxidation/polymerisation kinetics of Cu-F/Hs without ODA.

Context: pristine Cu-F/Hs target sample
Measurement source: 7 · 3.4 · Fig. 5B-C; Table S1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Km for Cu-F/Hs without ODA	61.51 uM	—	—	SI Table Exact Reported	4 · Table S1 · Table S1
Vmax for Cu-F/Hs without ODA	0.81 uM/s	—	—	SI Table Exact Reported	4 · Table S1 · Table S1

Michaelis-Menten kinetic assay by UV-vis

UV-visible and optical absorption spectroscopy

Cu-F/Hs with ODA in DA polymerisation mixture · Unknown

DA substrate oxidation/polymerisation kinetics of Cu-F/Hs in the presence of ODA.

Context: Cu-F/Hs plus ODA reaction mixture
Measurement source: 7 · 3.4 · Fig. 5B-C; Table S1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Km for Cu-F/Hs with ODAMarked as a best value within this paper	60.18 uM	—	—	SI Table Exact Reported	4 · Table S1 · Table S1
Vmax for Cu-F/Hs with ODAMarked as a best value within this paper	1.02 uM/s	—	—	SI Table Exact Reported	4 · Table S1 · Table S1

Michaelis-Menten kinetic assay by UV-vis

UV-visible and optical absorption spectroscopy

Cu-Fs powder/nanoparticles · Powder

DA substrate oxidation/polymerisation kinetics without ODA, fitted to V = Vmax[S]/(Km+[S]).

Context: pristine Cu-Fs control
Measurement source: 7 · 3.4 · Fig. 5B-C; Table S1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Km for Cu-Fs without ODA	100.24 uM	—	—	SI Table Exact Reported	4 · Table S1 · Table S1
Vmax for Cu-Fs without ODA	0.22 uM/s	—	—	SI Table Exact Reported	4 · Table S1 · Table S1

EIS PD-L1 aptamer concentration optimisation

Impedance spectroscopy

PD-L1 sandwich sensor on gold electrode · Electrode

Electrochemical impedance response measured for PD-L1 Apt concentrations from 0.1 to 2 uM before selecting 1 uM.

Geometry: gold-electrode biosensor surface
Context: composite sensor precursor interface
Measurement source: 3 · Supporting information · Fig. S2

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
0.1 uM PD-L1 Apt impedance change	about 160 ohm	—	error bar shown	Figure Axis Approximate	3 · Supporting information · Fig. S2
0.2 uM PD-L1 Apt impedance change	about 300 ohm	—	error bar shown	Figure Axis Approximate	3 · Supporting information · Fig. S2
0.5 uM PD-L1 Apt impedance change	about 540 ohm	—	error bar shown	Figure Axis Approximate	3 · Supporting information · Fig. S2
0.8 uM PD-L1 Apt impedance change	about 820 ohm	—	error bar shown	Figure Axis Approximate	3 · Supporting information · Fig. S2
1 uM PD-L1 Apt impedance changeMarked as a best value within this paper	about 1920 ohm	—	error bar shown	Figure Axis Approximate	3 · Supporting information · Fig. S2
2 uM PD-L1 Apt impedance change	about 1930 ohm	—	error bar shown	Figure Axis Approximate	3 · Supporting information · Fig. S2
Selected PD-L1 aptamer concentrationMarked as a best value within this paper	1 uM	—	—	Text Exact Reported	7 · 3.3 · Fig. S2