Sensing Application — Dual nanozyme based on ultrathin 2D conductive MOF nanosheets intergraded with gold nanoparticles for electrochemical biosensing of H2O2 in cancer cells

8 measurement groups · 23 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

Chronoamperometry / amperometric H2O2 sensing

Chronoamperometry

Au-NPs/Cu-HHTP-NSs/GCE · Electrode

Stepwise H2O2 additions in stirred 0.1 M PBS (pH 7.4) at -0.6 V; optimal potential selected from Figure S12.

Temperature: 298
Atmosphere: N2-saturated PBS per SI electrochemical methods
Geometry: three-electrode cell with Au-NPs/Cu-HHTP-NSs/GCE working electrode
Context: Au-loaded composite electrode
Measurement source: 6-7 · 3.3. Electrochemical sensing performances · Fig. 4d-f; Figure S12

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Response time to 95% steady-state current	95% within 3 s	—	—	Text Exact Reported	7 · 3.3. Electrochemical sensing performances · Fig. 4d-e
Figure 4f calibration R squared	R2 = 0.9984	—	—	Figure Axis Rounded Reported	7 · Figure 4 · Fig. 4f
Figure 4f calibration slope	y = 13.29164x - 0.5141	—	—	Figure Axis Rounded Reported	7 · Figure 4 · Fig. 4f
Linear range for H2O2 reduction-current responseMarked as a best value within this paper	50 nM-16.4 mM	—	—	Text Range	7 · 3.3. Electrochemical sensing performances · Fig. 4f
H2O2 limit of detectionMarked as a best value within this paper	5.6 nM by 3 sigma rules	—	—	Text Exact Reported	1, 7 · Abstract; 3.3. Electrochemical sensing performances · Fig. 4f
H2O2 sensing sensitivityMarked as a best value within this paper	188.1 uA cm-2 mM-1	—	—	Text Exact Reported	1, 7 · Abstract; 3.3. Electrochemical sensing performances · Fig. 4f

Live-cell amperometric H2O2 detection and DCFH-DA fluorescence validation

Au-NPs/Cu-HHTP-NSs/GCE · Electrode

NCM-460, SW-48 and HCT-116 cells stimulated with fMLP; amperometry at -0.6 V after 10 uM fMLP addition in solution containing 5.0 x 10^6 cells.

Atmosphere: Cell culture at 37 deg C with 5% CO2; electrochemical test atmosphere not specified
Geometry: three-electrode system in wells/plates with Au-NPs/Cu-HHTP-NSs/GCE
Context: application test using Au-loaded composite electrode
Measurement source: 2, 8 · 2.2-2.3; 3.4. Live cells detection · Fig. 5

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
HCT-116 fMLP-stimulated amperometric current change	0.52 uA	—	—	Text Exact Reported	8 · 3.4. Live cells detection · Fig. 5b
NCM-460 fMLP-stimulated amperometric current change	0.22 uA	—	—	Text Exact Reported	8 · 3.4. Live cells detection · Fig. 5b
No-cell fMLP control	No detectable current signal without cells	—	—	Text Qualitative	8 · 3.4. Live cells detection · Figure S13
SW-48 fMLP-stimulated amperometric current changeMarked as a best value within this paper	0.64 uA	—	—	Text Exact Reported	8 · 3.4. Live cells detection · Fig. 5b

Terephthalic-acid fluorescence assay for hydroxyl radical generation

Au-NPs/Cu-HHTP-NSs · Nanosheet

TA used as probe for OH generated from H2O2 by nanozymatic catalysis; emission monitored at 450 nm.

Geometry: solution assay
Context: pristine controls and Au-loaded composite
Measurement source: 5-6 · 3.2. Peroxidase-like activity investigation · Fig. 3e-f

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Relative OH generation efficiencyMarked as a best value within this paper	Au-NPs/Cu-HHTP-NSs much higher than Cu-HHTP-NSs and Cu-HHTP-bulk	—	—	Text Qualitative	5-6 · 3.2. Peroxidase-like activity investigation · Fig. 3e-f
TA-OH fluorescence emission	450 nm	—	—	Text Exact Reported	5 · 3.2. Peroxidase-like activity investigation · Fig. 3e-f

Michaelis-Menten kinetic fitting of TMB oxidation POD-like assay

Au-NPs/Cu-HHTP-NSs · Nanosheet

30 uL TMB, 100 uL H2O2 and 50 uL Au-NPs/Cu-HHTP-NSs mixed; H2O2 concentration 1.0 M for Figure S11/Table S1, TMB varied from 0 to nearly 1.75 mM.

Context: Composite sample; compared with literature nanozymes in Table S1.
Measurement source: 10-12 · Figure S11; Table S1 · Figure S11; Table S1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Michaelis-Menten Km for TMB substrate	0.3947 mM	0.3947 mM	—	SI Table Exact Reported	12 · Table S1 · Table S1
Michaelis-Menten Vmax for TMB oxidation	10.5x10-5 M min-1	0.000105 M min^-1	—	SI Table Exact Reported	12 · Table S1 · Table S1

Peroxidase-like TMB UV-Vis assay

UV-visible and optical absorption spectroscopy

Au-NPs/Cu-HHTP-NSs · Nanosheet

TMB/H2O2 colourimetric POD-like activity comparison; SI method used 30 uL TMB (20 mM), 100 uL H2O2 (60 mM), 50 uL Au-NPs/Cu-HHTP-NSs (200 ug mL-1), 15 min incubation.

Geometry: solution assay
Context: pristine controls and Au-loaded composite
Measurement source: SI text · Investigation of POD like activity of Au-NPs/Cu-HHTP-NSs · Fig. 3a-d; Figure S11

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
oxTMB absorbance wavelength	652 nm	—	—	Text Exact Reported	5 · 3.2. Peroxidase-like activity investigation · Fig. 3a
Relative POD-like activityMarked as a best value within this paper	Au-NPs/Cu-HHTP-NSs exhibited the maximum absorbance and much higher POD-like activity than Cu-HHTP-NSs and Cu-HHTP-bulk	—	—	Text Qualitative	5 · 3.2. Peroxidase-like activity investigation · Fig. 3a
Optimal POD-like pH rangeMarked as a best value within this paper	pH 3.2-4.8	—	—	Text Range	5 · 3.2. Peroxidase-like activity investigation · Fig. 3b

Electrode reproducibility and long-term stability amperometry

Au-NPs/Cu-HHTP-NSs/GCE · Electrode

Six electrodes tested for response to 0.5 mM H2O2; periodic response recording over 4 weeks.

Geometry: Au-NPs/Cu-HHTP-NSs/GCE
Context: Au-loaded composite electrode
Measurement source: 7 · 3.3. Electrochemical sensing performances · Fig. 4h-i

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Electrode-to-electrode reproducibility RSDMarked as a best value within this paper	3.1%	—	—	Text Exact Reported	7 · 3.3. Electrochemical sensing performances · Fig. 4h
Long-term current retention after 4 weeksMarked as a best value within this paper	93% of initial current value	—	—	Text Exact Reported	7 · 3.3. Electrochemical sensing performances · Fig. 4i

Amperometric interference/selectivity test

Chronoamperometry

Au-NPs/Cu-HHTP-NSs/GCE · Electrode

Response to 0.5 mM H2O2 and 1 mM interferents DA, UA, AA, Glu, L-cys, LA, H2S and GSH at -0.6 V.

Geometry: Au-NPs/Cu-HHTP-NSs/GCE
Context: Au-loaded composite electrode
Measurement source: 7 · 3.3. Electrochemical sensing performances · Fig. 4g

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Interference tolerance	0.5 mM H2O2 response almost not affected by 1 mM DA, UA, AA, Glu, L-cys, LA, H2S or GSH	—	—	Text Qualitative	7 · 3.3. Electrochemical sensing performances · Fig. 4g

SI comparison table of H2O2 sensor performance

Au-NPs/Cu-HHTP-NSs/GCE · Electrode

Table S2 lists linear range, sensitivity and LOD for Au-NPs/Cu-HHTP-NSs modified electrode and literature comparators.

Geometry: Modified glassy carbon electrode
Context: Composite electrode; this-work row extracted only, literature comparator rows not treated as first-hand evidence.
Measurement source: 12 · Table S2 · Table S2

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
SI Table S2 H2O2 linear range	50 nM-16 mM	—	—	SI Table Range	12 · Table S2 · Table S2
SI Table S2 H2O2 limit of detection	5.6 nM	5.6 nM	—	SI Table Exact Reported	12 · Table S2 · Table S2
SI Table S2 H2O2 sensitivity	188.1 uA mM-1 cm-2	188.1 uA cm^-2 mM^-1	—	SI Table Exact Reported	12 · Table S2 · Table S2