Other — Piezoelectric-mediated two-dimensional copper-based metal–organic framework for synergistic sonodynamic and cuproptosis-driven tumor therapy

3 measurement groups · 14 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

Hydrodynamic diameter and zeta potential monitoring

Dynamic light scattering and zeta potential

CM nanosheets · Nanosheet

CM dispersions in water, PBS (pH 7.4), and RPMI-1640 culture medium monitored over 14 days; zeta potential measured on day 0 and day 14.

Atmosphere: aqueous/physiological media
Context: PVP-coated Cu-2MI composite dispersion
Measurement source: p003 · 2.1 Synthesis and characterization · Fig. S4

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
CM hydrated diameter range over 14 days in physiological media	approximately 175-195 nm across water, PBS and 1640 medium over 14 days	—	visual range about 175-195 nm	Visual Estimate Approximate	S11 · Supplementary figures · Fig. S4a
CM zeta potential in RPMI-1640 medium at day 0	about -14.0 mV	—	visual estimate from bar plot	Visual Estimate Approximate	S11 · Supplementary figures · Fig. S4d
CM zeta potential in RPMI-1640 medium at day 14	about -14.2 mV	—	visual estimate from bar plot	Visual Estimate Approximate	S11 · Supplementary figures · Fig. S4d
CM zeta potential in PBS at day 0	about -14.0 mV	—	visual estimate from bar plot	Visual Estimate Approximate	S11 · Supplementary figures · Fig. S4c
CM zeta potential in PBS at day 14	about -14.5 mV	—	visual estimate from bar plot	Visual Estimate Approximate	S11 · Supplementary figures · Fig. S4c
CM zeta potential in water at day 0	about -14.5 mV	—	visual estimate from bar plot	Visual Estimate Approximate	S11 · Supplementary figures · Fig. S4b
CM zeta potential in water at day 14	about -14.8 mV	—	visual estimate from bar plot	Visual Estimate Approximate	S11 · Supplementary figures · Fig. S4b

CCK-8 cell viability, DCFH-DA, Calcein-AM/PI, JC-1, western blot and flow cytometry assays

CM nanosheets · Nanosheet

4T1 cells treated with CM and/or ultrasound; typical CM dose 60 ug mL^-1 and ultrasound 1 W cm^-2.

Temperature: 310
Atmosphere: humidified incubator with 5% CO2 for cell culture
Context: PVP-coated Cu-2MI composite in biomedical application
Measurement source: p006 · 2.4 In vitro therapeutic evaluation · Fig. 4

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
293T cell viability at 100 ug mL^-1 CM	remained above 80%	—	—	Text Range	p006 · 2.4 In vitro therapeutic evaluation · Fig. S14
Apoptosis rate under CM + USMarked as a best value within this paper	close to 70%	—	—	Text Approximate	p006 · 2.4 In vitro therapeutic evaluation · Fig. S16
4T1 cell viability under CM + US at 60 ug mL^-1Marked as a best value within this paper	24%	—	—	Text Exact Reported	p006 · 2.4 In vitro therapeutic evaluation · Fig. 4d

4T1 tumour-bearing mouse in vivo antitumour efficacy

CM nanosheets · Nanosheet

Four groups, n = 5: control, US, CM and CM + US; CM injected intravenously at 2 mg mL^-1, 100 uL; ultrasound 1 W cm^-2 for 4 min 12 h post-injection; 14-day treatment.

Atmosphere: in vivo
Context: PVP-coated Cu-2MI composite in biomedical application
Measurement source: 22. In Vivo Synergistic Therapy · Fig. 5

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Tumour inhibition rate for CM alone	30%	—	—	Text Exact Reported	p007 · 2.5 In vivo antitumor efficacy · Fig. 5g
Tumour inhibition rate for CM + USMarked as a best value within this paper	90%	—	—	Text Exact Reported	p007 · 2.5 In vivo antitumor efficacy · Fig. 5g
Hemolysis rate at 100 ug mL^-1 CM	below 5%	—	—	Text Range	p006 · 2.5 In vivo antitumor efficacy · Fig. S18
RNA-seq detected genes	27,549 genes	—	—	Text Exact Reported	p007 · 2.6 RNA sequencing analysis · Fig. 6