Synthesis · Piezoelectric-mediated two-dimensional copper-based metal–organic framework for synergistic sonodynamic and cuproptosis-driven tumor therapy

4 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Complete recipeSource: Both

Route 1: Other

2. Synthesis

Metal precursorsCu-2MI, 100 mg

SolventsMethanol-water mixture, 1:1 v/v, 20 mL

Additivespolyvinylpyrrolidone (PVP), 200 mg

Atmospherenot reported

Time1

Work-upCu-2MI dispersed in methanol-water; PVP added; stirred 1 h; centrifuged at 10000 rpm for 10 min; washed with water twice; redispersed in water; stored at 4 deg C.

Activationnot reported

Scalability context100 mg Cu-2MI coating batch; no scale-up discussion.

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	Cu-2MI	100 mg	2. Synthesis
Additive	PVP	200 mg	2. Synthesis
Solvent	methanol-water mixture (1:1 volume ratio)	20 mL	2. Synthesis

Complete recipeSource: SI

Route 2: Other

12. Cell Uptake · Fig. 4a-b

Metal precursorsCM methanol solution

Solventsmethanol; PBS before use

AdditivesRhodamine B, 3 mg

Atmospheredark

Time24

Work-up3 mg RhB mixed with 3 mL of 1 mg mL^-1 CM methanol solution and stirred in the dark for 24 h; centrifuged; washed with PBS; dispersed in PBS before use.

Activationnot reported

Scalability contextAnalytical cell-uptake labelling batch only.

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Additive	Rhodamine B (RhB)	3 mg	12. Cell Uptake · Fig. 4a-b
Other	CM methanol solution	3 mL · 1 mg mL^-1	12. Cell Uptake · Fig. 4a-b
Solvent	PBS	Not specified	12. Cell Uptake · Fig. 4a-b

Complete recipeSource: Both

Route 3: Solvothermal

2. Synthesis

Metal precursorsCu(NO3)2.3H2O, 2 mmol

Linker precursors2-methylimidazole (2-MI), 2 mmol

SolventsMethanol-water mixture, 1:1 v/v; 25 mL for Cu solution and 25 mL for 2-MI solution

Atmospherenot reported

Time0.1667

Work-upSolution B added to solution A; stirred 10 min; blue product centrifuged at 10000 rpm for 10 min; washed multiple times with anhydrous ethanol; redispersed in anhydrous ethanol; stored at 4 deg C.

Activationnot reported

Scalability contextMillimole-scale batch in 50 mL total solvent; no scale-up discussion.

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Cu(NO3)2.3H2O	2 mmol	2. Synthesis
Linker	2-methylimidazole (2-MI)	2 mmol	2. Synthesis
Solvent	methanol-water mixture (1:1 volume ratio)	25 mL + 25 mL	2. Synthesis

Complete recipeSource: SI

Route 4: Other

2. Synthesis

Metal precursorsZn(NO3)2.6H2O, 1 mmol

Linker precursors2-methylimidazole (2-MI), 4 mmol

SolventsMethanol-water mixture, 1:1 v/v; 25 mL for Zn solution and 25 mL for 2-MI solution

Atmospherenot reported

Time1

Work-upSolution B added to solution A; stirred 60 min; white product centrifuged at 10000 rpm for 10 min; washed multiple times with anhydrous ethanol; redispersed in anhydrous ethanol; stored at 4 deg C.

Activationnot reported

Scalability contextMillimole-scale benchmark synthesis; no scale-up discussion.

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Zn(NO3)2.6H2O	1 mmol	2. Synthesis
Linker	2-methylimidazole (2-MI)	4 mmol	2. Synthesis
Solvent	methanol-water mixture (1:1 volume ratio)	25 mL + 25 mL	2. Synthesis