Sensing Application — An Enzyme-Encapsulated Metal-Organic Frameworks Nanomesh Biosensor for Salivary Glucose Detection

8 measurement groups · 22 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

Correlation of salivary biosensor readings with commercial blood glucose meter

CNT/GOx@ZIF-8 biosensor electrode · Electrode

Saliva samples and blood glucose readings collected during daily activities; Pearson correlation calculated from 21 samples of three volunteers.

Geometry: SPCE-based CNT/GOx@ZIF-8 biosensor and Roche Accu-Chek blood glucose meter.
Context: Application validation of target composite biosensor.
Measurement source: p006 · 2.6. Practical Evaluation · Figure 6d-f

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Pearson correlation with blood glucose meterMarked as a best value within this paper	Pearson's r = 0.70923	—	21 samples from three volunteers	Text Exact Reported	p006 · 2.6. Practical Evaluation · Figure 6f
Blood-correlation sample count	21 samples	3 volunteers	—	Text Exact Reported	p006 · 2.6. Practical Evaluation · Figure 6f

Amperometric i-t calibration for glucose sensing

CNT/GOx@ZIF-8 biosensor electrode · Electrode

Successive glucose additions to 0.1 M PBS (pH 7.4) at -0.45 V; final glucose concentration from 10 uM to 2 mM; calibration reported from 10 to 750 uM.

Geometry: SPCE-based CNT/GOx@ZIF-8 biosensor.
Context: Target composite biosensor.
Measurement source: p006 · Figure caption · Figure 4c,d

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Calibration slope, 200-750 uM glucose	I(uA) = 0.0062 Cglucose(uM) + 0.8554, R2 = 0.9978	—	R2 = 0.9978	Text Exact Reported	p005 · 2.4. Electrochemical Characterization · Figure 4d
Calibration slope, 10-200 uM glucose	I(uA) = 0.0094 Cglucose(uM) + 0.2575, R2 = 0.9974	—	R2 = 0.9974	Text Exact Reported	p005 · 2.4. Electrochemical Characterization · Figure 4d
Linear calibration lower bound	10 uM	0.01 mM	—	Caption Exact Reported	p006 · Figure caption · Figure 4d
Linear calibration upper bound	750 uM	0.75 mM	—	Caption Exact Reported	p006 · Figure caption · Figure 4d
Limit of detection for glucoseMarked as a best value within this paper	16.57 uM	0.01657 mM	—	Text Exact Reported	p005 · 2.4. Electrochemical Characterization · Figure 4d
Glucose sensitivityMarked as a best value within this paper	86.86 uA mM-1 cm-2	—	—	Text Exact Reported	p005 · 2.4. Electrochemical Characterization · Figure 4d

Human saliva glucose assay comparison

CNT/GOx@ZIF-8 biosensor electrode · Electrode

40 uL human saliva dropped on sensing interface; amperometric i-t at -0.45 V for 60 s; compared with commercial salivary glucose content assay kit.

Geometry: SPCE-based CNT/GOx@ZIF-8 biosensor.
Context: Target composite biosensor tested on real saliva samples.
Measurement source: p006 · 2.6. Practical Evaluation · Figure 6a-c

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Accuracy versus commercial salivary glucose assay kitMarked as a best value within this paper	93.18%	—	—	Text Exact Reported	p006 · 2.6. Practical Evaluation · Figure 6b,c
Pearson correlation versus salivary assay kitMarked as a best value within this paper	Pearson's r = 0.9808	—	N = 3 in plot	Figure Axis Exact Reported	p008 · Figure 6 · Figure 6c

Batch-to-batch reproducibility by amperometric response

Chronoamperometry

CNT/GOx@ZIF-8 biosensor electrode · Electrode

Three batches of biosensors measured in glucose solutions of 0, 50, 100, 200 and 300 uM.

Geometry: SPCE-based CNT/GOx@ZIF-8 biosensor.
Context: Target composite biosensor; N=3.
Measurement source: p005 · 2.5. Selectivity, Reproducibility, and Stability · Figure 5c,d

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Batch reproducibility coefficient of variationMarked as a best value within this paper	1.32%	—	—	Text Exact Reported	p005 · 2.5. Selectivity, Reproducibility, and Stability · Figure 5c,d

Amperometric interference/selectivity test

Chronoamperometry

CNT/GOx@ZIF-8 biosensor electrode · Electrode

Successive additions of 100 uM glucose, 4 mM urea, 5 uM ascorbic acid, 5 uM L-cysteine, 100 uM sucrose and 100 uM glucose.

Geometry: SPCE-based CNT/GOx@ZIF-8 biosensor.
Context: Target composite biosensor.
Measurement source: p007 · Figure caption · Figure 5a,b

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Initial glucose current responseMarked as a best value within this paper	approximately 0.43 uA	—	N = 3	Figure Axis Approximate	p007 · Figure 5 · Figure 5b
Sucrose interferent current response	approximately 0.02 uA	—	N = 3	Figure Axis Approximate	p007 · Figure 5 · Figure 5b
Urea interferent current response	approximately 0.05 uA	—	N = 3	Figure Axis Approximate	p007 · Figure 5 · Figure 5b

Amperometric current-response optimisation of biosensor preparation conditions

Chronoamperometry

CNT/GOx@ZIF-8 biosensor electrode · Electrode

Figure S1 varies 2-MI/Zn2+ ratio, Zn2+ concentration and CNT concentration; D-value is background-subtracted current response in PBS for glucose detection.

Geometry: SPCE-based CNT/GOx@ZIF-8 biosensor during optimisation.
Context: Target composite biosensor preparation variables.
Measurement source: SI p.2 · Figure S1 caption · Figure S1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
CNT concentration giving maximum responseMarked as a best value within this paper	[CNT] = 16.5 mg/mL gave approximately 3.5 uA D-value response at 500 uM glucose, similar to 22 mg/mL	3.5 uA D-value at 500 uM glucose	N = 3; visual estimate	Figure Axis Approximate	SI p.2 · Figure S1 · Figure S1f
Best 2-MI/Zn2+ optimisation ratioMarked as a best value within this paper	2-MI/Zn2+ = 5 gave the largest D-value response at 300 uM glucose, approximately 2.2 uA	2.2 uA D-value at 300 uM glucose	N = 3; visual estimate	Figure Axis Approximate	SI p.2 · Figure S1 · Figure S1b
Zn2+ concentration giving near-maximum response	[Zn] = 48 mM gave approximately 2.1 uA D-value response at 300 uM glucose; 30 mM was slightly higher or comparable	2.1 uA D-value at 300 uM glucose	N = 3; visual estimate	Figure Axis Approximate	SI p.2 · Figure S1 · Figure S1d

Analytical-performance comparison table

CNT/GOx@ZIF-8 biosensor electrode · Electrode

Table S1 compares linear range, sensitivity and LOD for this CNT/GOx@ZIF-8/Nafion biosensor against literature biosensors.

Geometry: CNT/GOx@ZIF-8/Nafion electrode.
Context: This-work row of SI comparison table only; literature comparator rows not extracted as first-hand evidence.
Measurement source: SI pp.3-4 · Table S1 · Table S1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
CNT/GOx@ZIF-8/Nafion linear range from Table S1	0.01~0.75 mM	0.01 mM lower bound	—	SI Table Exact Reported	SI pp.3-4 · Table S1 · Table S1
CNT/GOx@ZIF-8/Nafion LOD from Table S1Marked as a best value within this paper	16.57 uM	0.01657 mM	—	SI Table Exact Reported	SI pp.3-4 · Table S1 · Table S1
CNT/GOx@ZIF-8/Nafion sensitivity from Table S1Marked as a best value within this paper	86.86 uA mM-1 cm-2	—	—	SI Table Exact Reported	SI pp.3-4 · Table S1 · Table S1

Long-term stability and shelf-life amperometric response

Chronoamperometry

CNT/GOx@ZIF-8 biosensor electrode · Electrode

Biosensors stored in 0.1 M PBS (pH 7.4) at room temperature for usage stability; separate storage at 4 degC for shelf-life.

Temperature: room temperature and 277 K storage subsets
Geometry: SPCE-based CNT/GOx@ZIF-8 biosensor.
Context: Target composite biosensor.
Measurement source: p006 · 2.5. Selectivity, Reproducibility, and Stability · Figure 5e,f

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Shelf-life at 4 degC with small response lossMarked as a best value within this paper	14 days; current responses slightly decreased (<6%)	6 % response decrease	<6%	Text Rounded Reported	p006 · 2.5. Selectivity, Reproducibility, and Stability · Figure 5f
Working life with at least 80% responseMarked as a best value within this paper	16 days; up to 80% of first-day response	80 % first-day response	—	Text Rounded Reported	p006 · 2.5. Selectivity, Reproducibility, and Stability · Figure 5e