Spectroscopy — An Enzyme-Encapsulated Metal-Organic Frameworks Nanomesh Biosensor for Salivary Glucose Detection

Measurement evidence

Spectroscopy

An Enzyme-Encapsulated Metal-Organic Frameworks Nanomesh Biosensor for Salivary Glucose Detection · Wang M., Wang H., Cheng J. · Advanced Materials Technologies · 2024 · 2301678

3 measurement groups · 7 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

GOx Michaelis-Menten and Lineweaver-Burk kinetic-control analysis by UV-vis at 652 nm

GOx electrode / GOx in solution · Electrode

GOx control measured with TMB chromogenic curves at glucose concentrations of 0.5, 1, 4 and 8 mM; kinetic fits shown in Figure S2c,d.

Geometry
Solution UV-vis assay.
Context
GOx-only control for comparison with CNT/GOx@ZIF-8 catalysis.
Measurement source
SI p.3 · Figure S2 caption · Figure S2
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
GOx solution comparison KM2.60 mMText
Exact Reported
p004 · 2.3. Catalytic Properties of CNT/GOx@ZIF-8 · Figure 3d,e; SI Figure S2c,d
GOx Lineweaver-Burk fit equationy = 2.4352*10^3 x + 0.9349*10^3934.9 intercept in plotted Lineweaver-Burk unitsFigure Axis
Rounded Reported
SI p.3 · Figure S2 · Figure S2d
GOx solution comparison Vmax1.070 uM s-1Text
Exact Reported
p004 · 2.3. Catalytic Properties of CNT/GOx@ZIF-8 · Figure 3d,e; SI Figure S2c,d

Michaelis-Menten and Lineweaver-Burk kinetic analysis by UV-vis at 652 nm

CNT/GOx@ZIF-8 nanomesh · Powder

PBS containing glucose at 0.25, 0.5, 1 and 2 mM with TMB 0.5 mM, HRP 2 ug mL-1 and CNT/GOx@ZIF-8 100 ug mL-1.

Context
Composite target compared with GOx in solution.
Measurement source
p009 · Analysis of Catalytic Performance · Figure 3d,e
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
CNT/GOx@ZIF-8 Michaelis constant KMMarked as a best value within this paper2.32 mMText
Exact Reported
p004 · 2.3. Catalytic Properties of CNT/GOx@ZIF-8 · Figure 3d,e
CNT/GOx@ZIF-8 Vmax0.644 uM s-1Text
Exact Reported
p004 · 2.3. Catalytic Properties of CNT/GOx@ZIF-8 · Figure 3d,e

UV-vis TMB colorimetric catalytic assay

CNT/GOx@ZIF-8 nanomesh · Powder

Mixture containing 200 ug mL-1 CNT/GOx@ZIF-8, 0.5 mM TMB, 2 ug mL-1 HRP and 0.5 mM glucose; spectra recorded from 300 to 750 nm after colour change.

Context
Composite catalytic activity compared with CNT, ZIF-8, GOx, CNT@ZIF-8, CNT/GOx and GOx@ZIF-8 controls.
Measurement source
p004 · 2.3. Catalytic Properties of CNT/GOx@ZIF-8 · Figure 3a,b
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
CNT/GOx@ZIF-8 catalytic activity retention durationMarked as a best value within this paperheld up to 10 days; peak value remained above 80%80 % peak value retention>80%Text
Rounded Reported
p004 · 2.3. Catalytic Properties of CNT/GOx@ZIF-8 · Figure 3f
Oxidized TMB absorbance bandscharacteristic absorbances at 370 and 652 nmText
Rounded Reported
p004 · 2.3. Catalytic Properties of CNT/GOx@ZIF-8 · Figure 3a-c