Synthesis · An Enzyme-Encapsulated Metal-Organic Frameworks Nanomesh Biosensor for Salivary Glucose Detection

2 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Complete recipeSource: Main

p009 · Fabrication of CNT/GOx@ZIF-8 Biosensor · Figure 1b

Solventsas-prepared aqueous CNT/GOx@ZIF-8 dispersion; Nafion solution

Additives1 uL 5% Nafion solution

Atmosphereambient air, inferred from room-temperature drying

Temperatureroom temperature

Time1 h drying; 1 h PBS immersion before characterisation

Substrate orientationworking electrode of screen-printing carbon electrode (SPCE)

Work-upDried at room temperature, rinsed with ultrapure water three times, immersed in PBS for 1 h.

ActivationPBS immersion before CHI760 electrochemical characterisation

Scalability contextDrop-casting 6 uL on SPCE is compatible with disposable electrode fabrication, but no manufacturing-scale process was reported.

Show 4 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	as-prepared CNT/GOx@ZIF-8 solution	9 uL	p009 · Fabrication of CNT/GOx@ZIF-8 Biosensor · Figure 1b
Additive	Nafion solution	1 uL · 5%	p009 · Fabrication of CNT/GOx@ZIF-8 Biosensor · Figure 1b
Other	drop-cast mixture applied to SPCE	6 uL	p009 · Fabrication of CNT/GOx@ZIF-8 Biosensor · Figure 1b
Solvent	PBS	immersion for 1 h	p009 · Fabrication of CNT/GOx@ZIF-8 Biosensor · Figure 1b

Complete recipeSource: Main

p009 · Synthesis of CNT/GOx@ZIF-8

Metal precursorsZn(NO3)2.6H2O, 14.8 mg in 1 mL ultrapure water

Linker precursors2-methylimidazole (2-MI), 21.44 mg in 1 mL ultrapure water

Solventsultrapure water

Additives200 uL 10 wt% CNT solution; 20 mg glucose oxidase (GOx)

Atmospherenot reported

Temperatureroom temperature

Time0.083 h ultrasonic mixing for each precursor solution; 0.083 h stirring after CNT addition; 3 h after adding zinc solution

Work-upWashed three times with ultrapure water by centrifugation at 6000 rpm for 6 min; dispersed into 5 mL ultrapure water.

Scalability contextSmall-batch aqueous room-temperature synthesis; no scale-up data reported.

Show 5 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Zn(NO3)2.6H2O	14.8 mg	p009 · Synthesis of CNT/GOx@ZIF-8
Linker	2-Methylimidazole (2-MI)	21.44 mg	p009 · Synthesis of CNT/GOx@ZIF-8
Solvent	ultrapure water	1 mL for Zn solution; 1 mL for 2-MI solution; wash solvent; final dispersion 5 mL	p009 · Synthesis of CNT/GOx@ZIF-8
Additive	carbon nanotube solution (CNTs)	200 uL · 10 wt%	p009 · Synthesis of CNT/GOx@ZIF-8
Additive	glucose oxidase (GOx)	20 mg	p009 · Synthesis of CNT/GOx@ZIF-8