Sensing Application — Ruthenium(II) complex-grafted conductive metal-organic frameworks with conductivity- and confinement-enhanced electrochemiluminescence for ultrasensitive biosensing application

5 measurement groups · 20 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

ECL response control with and without AuNP decoration

Fc-S1/HT/aptamer/AuNPs/Ru@Ni3(HITP)2/GCE biosensor · Electrode

Compared Fc-S1/HT/aptamer/AuNPs/Ru@Ni3(HITP)2/GCE with Fc-S1/HT/aptamer/Ru@Ni3(HITP)2/GCE to test whether pi-pi interactions alone immobilise the aptamer/Fc-S1 quenching system.

Geometry: Modified GCE electrode
Context: Final biosensor architecture versus no-AuNP control.
Measurement source: SI text · S-17 · Fig. S12

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
ECL signal with AuNP-mediated aptamer/Fc-S1 quenching	1071 a.u.	—	—	Text Exact Reported	SI text · S-17 · Fig. S12
ECL signal without AuNP anchoring	14138 a.u.	—	—	Text Exact Reported	SI text · S-17 · Fig. S12

Optimisation of ECL biosensor conditions

Fc-S1/HT/aptamer/AuNPs/Ru@Ni3(HITP)2/GCE biosensor · Electrode

Optimised Ru@Ni3(HITP)2 concentration, Fc-S1 concentration, and target TB/Exo I incubation time.

Geometry: Final biosensor GCE electrode
Context: Final biosensor application testing.
Measurement source: SI text · S-16 · Fig. S11

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Optimal Fc-S1 concentration	2 uM	—	—	Text Exact Reported	SI text · S-16 · Fig. S11B
Optimal target TB/Exo I incubation time	150 min	—	—	Text Exact Reported	SI text · S-16 · Fig. S11C
Optimal Ru@Ni3(HITP)2 dispersion concentration	1 mg/mL	—	—	Text Exact Reported	SI text · S-16 · Fig. S11A

Selectivity, reproducibility and cyclic stability ECL tests

Fc-S1/HT/aptamer/AuNPs/Ru@Ni3(HITP)2/GCE biosensor · Electrode

Selectivity versus 100 fM MUC1, beta2-MG, AFP, cholesterol and UA; reproducibility from five parallel 1 pM TB tests; stability under 20 continuous cycles at 100 pM and 1 fM TB.

Geometry: Final biosensor GCE electrode
Context: Final biosensor application testing.
Measurement source: p006 · 3.6 · Fig. 5

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Reproducibility RSD for 1 pM TB	0.93%	—	five parallel tests	Text Exact Reported	p006 · 3.6 · Fig. 5B
Stability RSD at 100 pM TB over 20 cyclesMarked as a best value within this paper	0.47%	—	20 cycles	Text Exact Reported	p006 · 3.6 · Fig. 5C
Stability RSD at 1 fM TB over 20 cycles	1.77%	—	20 cycles	Text Exact Reported	p006 · 3.6 · Fig. 5D

ECL thrombin calibration

Fc-S1/HT/aptamer/AuNPs/Ru@Ni3(HITP)2/GCE biosensor · Electrode

Constructed biosensor incubated with target TB concentrations under optimised conditions; ECL detected in 2 mL PBS (0.1 M, pH 7.0) containing 5 uL TPrA, 0.2-1.25 V, 0.3 V/s.

Geometry: Final biosensor GCE electrode
Context: Final Ru@Ni3(HITP)2/AuNP/aptamer/Fc-S1 biosensor.
Measurement source: SI text · S-3.5; S-19 · Fig. 4

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Calibration intercept	I = 8833.3 + 2495.1 lg[c/(pM)]	—	—	Text Exact Reported	p005 · 3.5 · Fig. 4B
Calibration slope	2495.1	—	—	Text Exact Reported	p005 · 3.5 · Fig. 4B
Calibration correlation coefficientMarked as a best value within this paper	R = 0.9996	—	—	Text Exact Reported	p005 · 3.5 · Fig. 4B
Thrombin linear range upper boundMarked as a best value within this paper	1 fM to 1 nM	—	—	Text Exact Reported	p005 · 3.5 · Fig. 4A
Thrombin linear range lower boundMarked as a best value within this paper	1 fM to 1 nM	—	—	Text Exact Reported	p005 · 3.5 · Fig. 4A
Thrombin detection limitMarked as a best value within this paper	0.62 fM (S/N = 3)	—	—	Text Exact Reported	p005 · 3.5 · Fig. 4B
Blank ECL intensity for LOD calculation	IB = 820 a.u.	—	SB = 3.33	Text Exact Reported	SI text · S-19
LOD threshold signal IL	IL = 829.99	—	—	Calculated From Reported Exact Reported	SI text · S-19

Human serum spike recovery assay

Fc-S1/HT/aptamer/AuNPs/Ru@Ni3(HITP)2/GCE biosensor · Electrode

Recovery assays measuring TB in human serum samples with five detection times per sample.

Geometry: Final biosensor GCE electrode
Context: Final biosensor application testing.
Measurement source: p006 · 3.7 · Table 2

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Human serum recovery at 0.01 pM added TB	Found 0.00997 pM; recovery 99.7%; RSD 2.50%	—	RSD 2.50%; n=5 detection times	Table Exact Reported	p006 · 3.7 · Table 2
Human serum recovery at 1 pM added TB	Found 1.030 pM; recovery 103.0%; RSD 2.64%	—	RSD 2.64%; n=5 detection times	Table Exact Reported	p006 · 3.7 · Table 2
Human serum recovery at 100 pM added TB	Found 104.1 pM; recovery 104.1%; RSD 4.86%	—	RSD 4.86%; n=5 detection times	Table Exact Reported	p006 · 3.7 · Table 2
Human serum recovery at 1000 pM added TB	Found 1028 pM; recovery 102.8%; RSD 3.23%	—	RSD 3.23%; n=5 detection times	Table Exact Reported	p006 · 3.7 · Table 2