Sensing Application — A conductive metal-organic framework-modified electrode for sensitive electrochemiluminescent detection of cardiac Troponin I

4 measurement groups · 11 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

SPCE/Cu3(HHTP)2/Ab/BSA immunosensor · Electrode

Optimised ECL conditions; cTnI additions over 0-80 pg/mL in PBS containing 100 uM [Ru(bpy)3]2+, n = 3.

Geometry: SPCE/Cu3(HHTP)2/Ab/BSA immunosensor
Context: final immunosensor
Measurement source: p008-p009 · 3.7 Analytical performance · Figure 9; Table 1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Clinical threshold comparison for early AMI diagnosis	0.4 ng/mL or 400 pg/mL	0.4 ng/mL	—	Text Exact Reported	p008 · 3.7 Analytical performance
Calibration correlation coefficientMarked as a best value within this paper	R = 0.99	—	—	Text Exact Reported	p008 · 3.7 Analytical performance · Figure 9B
cTnI linear calibration rangeMarked as a best value within this paper	0-80 pg/mL	—	range	Text Range	p008-p009 · 3.7 Analytical performance · Figure 9; Table 1
Limit of detection for cTnIMarked as a best value within this paper	10.23 +/- 1.06 pg/mL	—	+/- 1.06 pg/mL	Text Exact Reported	p008-p009 · 3.7 Analytical performance; Table 1 · Table 1
Limit of quantification for cTnIMarked as a best value within this paper	30.98 +/- 3.20 pg/mL	—	+/- 3.20 pg/mL	Text Exact Reported	p008 · 3.7 Analytical performance
Calibration regression intercept	21.64 a.u.	—	—	Text Exact Reported	p008 · 3.7 Analytical performance · Figure 9B
Calibration regression slopeMarked as a best value within this paper	y = 0.85X + 21.64	—	—	Text Exact Reported	p008 · 3.7 Analytical performance · Figure 9B

SPCE/Cu3(HHTP)2 · Electrode

Drop-casting suspensions of different Cu3(HHTP)2 loadings; ECL intensity monitored.

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Optimal Cu3(HHTP)2 dispersion concentrationMarked as a best value within this paper	2 mg/mL selected as optimal c-MOF loading	—	—	Text Exact Reported	p007 · 3.6 Optimization · Figure 8A

SPCE/Cu3(HHTP)2/Ab/BSA immunosensor · Electrode

Solution pH varied between 6.0 and 8.0; pH 7.0 chosen for immunoassays despite peak at pH 8.0.

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
pH at maximum ECL response	pH 8.0 peak	—	—	Text Exact Reported	p007-p008 · 3.6 Optimization · Figure 8B
pH selected for immunoassayMarked as a best value within this paper	pH 7.0 preferred for immunoassays	—	—	Text Exact Reported	p007-p008 · 3.6 Optimization · Figure 8B

SPCE/Cu3(HHTP)2/Ab/BSA immunosensor · Electrode

[Ru(bpy)3]2+ concentration varied from 1 uM to 150 uM; maximum at 100 uM.

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Optimal [Ru(bpy)3]2+ concentrationMarked as a best value within this paper	100 uM maximum ECL intensity	—	—	Text Exact Reported	p008 · 3.6 Optimization · Figure 8C