Synthesis evidence

A conductive metal-organic framework-modified electrode for sensitive electrochemiluminescent detection of cardiac Troponin I

Murray A., Valenti G., Baker P. · Electrochimica Acta · 2026 · 148350

4 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Complete recipeSource: Main

Route 1: Solvothermal

p002 · 2.3 Synthesis of Cu3(HHTP)2

Metal precursorsCu2(OAc)4.H2O, 17.9 mg (0.090 mmol)
Linker precursorsHHTP, 14.2 mg (0.044 mmol)
Solvents8 mL ultrapure H2O; 1.0 mL DMF added dropwise
Atmospherenot specified; capped glass vial
Temperature80
Time12
Work-upCentrifuged; washed three times with H2O, ethanol, and acetone.
ActivationDried in a vacuum oven at 80 deg C for 24 h.
Show 4 structured reagent records
RoleReagentAmount / concentrationSource
Metal SourceCu2(OAc)4.H2O17.9 mg (0.090 mmol)p002 · 2.3 Synthesis of Cu3(HHTP)2
LinkerHHTP14.2 mg (0.044 mmol)p002 · 2.3 Synthesis of Cu3(HHTP)2
Solventultrapure H2O8 mLp002 · 2.3 Synthesis of Cu3(HHTP)2
SolventDMF1.0 mLp002 · 2.3 Synthesis of Cu3(HHTP)2
Complete recipeSource: Main

Route 2: Other

p002 · 2.4 Fabrication

SolventsPBS rinse/blocking medium
AdditivescTnI antibody; BSA
Atmospherenot specified
Temperature4
Time1 h Ab incubation; 0.5 h BSA incubation
Substrate orientationSPCE/Cu3(HHTP)2 electrode
Work-upSamples gently rinsed with PBS after each biomolecule incubation step.
Show 3 structured reagent records
RoleReagentAmount / concentrationSource
Othermonoclonal cTnI antibody (Ab)1 ug/mLp002 · 2.4 Fabrication
OtherBSA in PBS1 wt %p002 · 2.4 Fabrication
SolventPBSNot specifiedp002 · 2.4 Fabrication
Complete recipeSource: Main

Route 3: Drop Cast

p002 · 2.4 Fabrication

Metal precursorsCu3(HHTP)2 powder already synthesised
SolventsH2O dispersion
Atmosphereambient laboratory atmosphere; not otherwise specified
Temperatureroom temperature
Timeovernight drying
Substrate orientationscreen-printed carbon electrode
Work-upAllowed to dry at room temperature overnight.
Show 2 structured reagent records
RoleReagentAmount / concentrationSource
OtherCu3(HHTP)215 uL dispersion · 2 mg/mL in H2Op002 · 2.4 Fabrication
SolventH2ONot specifiedp002 · 2.4 Fabrication
Partial recipeSource: SI

Route 4: Drop Cast

p002 · Figure S1 note · Figure S1

Linker precursorsHHTP
Solventsacetone
Atmospherenot specified
Substrate orientationSPCE
Work-upDrop-coated at SPCE to form a film.
Show 2 structured reagent records
RoleReagentAmount / concentrationSource
LinkerHHTPNot specifiedp002 · Figure S1 note · Figure S1
SolventacetoneNot specifiedp002 · Figure S1 note · Figure S1