Sensing Application — Sub-Femtomolar, Label-Free Small-Molecule Sensing with Nanoarchitectonic Metal-Organic Frameworks

6 measurement groups · 26 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

five-parameter logistic fit of cortisol calibration curve

cortisol-aptamer-functionalised CuHITP EG-FET sensor · Electrode

SI Figure S10 fits response versus log[cortisol, M] using a Logistic5 model.

Atmosphere: PBS 0.01X electrolyte, ambient measurement conditions
Geometry: CuHITP/Cu(OH)2 extended-gate FET with cortisol aptamer
Context: aptamer-functionalised conductive-MOF sensor
Measurement source: 9 · Supporting Information · Figure S10

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
5PL adjusted R-square	0.9958	—	—	SI Table Exact Reported	9 · Supporting Information · Figure S10
5PL Amax	30.59081 +/- 0.8292	—	+/- 0.8292	SI Table Exact Reported	9 · Supporting Information · Figure S10
5PL Amin	-6.77631 +/- 14.73464	—	+/- 14.73464	SI Table Exact Reported	9 · Supporting Information · Figure S10
5PL h steepness parameter	-5.5984 +/- 2.12648	—	+/- 2.12648	SI Table Exact Reported	9 · Supporting Information · Figure S10
5PL R-square COD	0.99733	—	—	SI Table Exact Reported	9 · Supporting Information · Figure S10
5PL s asymmetry parameter	0.55097 +/- 0.9933	—	+/- 0.9933	SI Table Exact Reported	9 · Supporting Information · Figure S10
5PL x0 inflection parameter	-10.23362 +/- 3.34865	—	+/- 3.34865	SI Table Exact Reported	9 · Supporting Information · Figure S10

label-free EG-FET cortisol sensing in 0.01X PBS

Field-effect transistor measurements

cortisol-aptamer-functionalised CuHITP EG-FET sensor · Electrode

Gate voltage swept from -1.5 to 0 V in 0.025 V increments while cortisol concentration increased from 0.1 fM to 10 uM in tenfold steps; n = 3 for calibration.

Atmosphere: 0.01X PBS electrolyte
Geometry: aptamer-functionalised CuHITP extended gate connected to commercial p-channel MOSFET
Context: MBS/cortisol-aptamer-functionalised conductive-MOF heterostructure
Measurement source: 7 · 2.3.2 Cortisol Sensing · Figure 6b,c

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
tested cortisol concentration range	0.1 fM to 10 uM in tenfold steps	—	—	Text Exact Reported	7 · 2.3.2 Cortisol Sensing · Figure 6b,c
cortisol dynamic rangeMarked as a best value within this paper	11 orders of magnitude	—	—	Text Exact Reported	8 · 2.3.2 Cortisol Sensing · Figure 6c
cortisol limit of detectionMarked as a best value within this paper	0.1 fM	1e-16 M	—	Text Rounded Reported	1 · Abstract
maximum cortisol calibration response	about 30 mV at log[cortisol,M] = -5	—	visual estimate	Visual Estimate Approximate	8 · Figure 6 · Figure 6c
gate voltage sweep range	-1.5 to 0 V	—	—	Text Exact Reported	7 · 2.3.2 Cortisol Sensing · Figure 6b
gate voltage sweep increment	0.025 V	25 mV	—	Text Exact Reported	7 · 2.3.2 Cortisol Sensing · Figure 6b

specificity/selectivity test against interfering biomolecules

cortisol-aptamer-functionalised CuHITP EG-FET sensor · Electrode

Normalized response toward cortisol 100 pM, glucose 10 nM, serotonin 10 nM and dopamine 10 nM; n = 3.

Atmosphere: PBS electrolyte, exact concentration not restated in caption
Geometry: cortisol-aptamer-functionalised CuHITP EG-FET
Context: Application sample with specific aptamer
Measurement source: 8 · Figure 6 caption · Figure 6d

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
normalised response to cortisolMarked as a best value within this paper	100% at cortisol 100 pM	1 fraction	—	Figure Axis Exact Reported	8 · Figure 6 · Figure 6d
normalised response to dopamine	about 8% at dopamine 10 nM	0.08 fraction	visual estimate	Visual Estimate Approximate	8 · Figure 6 · Figure 6d
normalised response to glucose	about 5% at glucose 10 nM	0.05 fraction	visual estimate	Visual Estimate Approximate	8 · Figure 6 · Figure 6d
normalised response to serotonin	about 14% at serotonin 10 nM	0.14 fraction	visual estimate	Visual Estimate Approximate	8 · Figure 6 · Figure 6d

MBS concentration optimisation by P count map and cortisol calibration

cortisol-aptamer-functionalised CuHITP EG-FET sensor · Electrode

SI Figure S13 compares aptamer-functionalised samples using 0.1, 1, and 10 mM MBS.

Geometry: aptamer-functionalised CuHITP/Cu(OH)2 EG-FET sensor
Context: MBS/aptamer-functionalised conductive-MOF sensor
Measurement source: 13 · Supporting Information · Figure S13

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
0.1 mM MBS maximum cortisol response	about 16 mV at high cortisol concentration	—	—	Visual Estimate Approximate	13 · Supporting Information · Figure S13d
10 mM MBS plateau response	about 13 mV plateau at high cortisol concentration	—	—	Visual Estimate Approximate	13 · Supporting Information · Figure S13d
best MBS concentration by visual calibration responseMarked as a best value within this paper	1 mM MBS gives the largest high-concentration response among 0.1, 1 and 10 mM	—	—	Visual Estimate Approximate	13 · Supporting Information · Figure S13d
1 mM MBS maximum cortisol response	about 24 mV at high cortisol concentration	—	—	Visual Estimate Approximate	13 · Supporting Information · Figure S13d

EG-FET pH sensing via threshold-voltage shift

Field-effect transistor measurements

CuHITP/Cu(OH)2 extended-gate electrode · Electrode

Sensing electrode exposed to pH 11 to pH 4 in one-unit steps, with Milli-Q rinse between measurements; pH 7 retested after pH 11 and pH 4 exposure.

Atmosphere: aqueous pH solutions
Geometry: CuHITP extended-gate FET with Ag/AgCl gate control
Context: Unaptamerised CuHITP/Cu(OH)2 EG electrode using intrinsic -NH- groups
Measurement source: 4 · 2.2 Electrical Characterization and pH Sensing · Figure 4

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
pH calibration equation	y = -23x + 232.67	—	—	Figure Axis Exact Reported	6 · Figure 4 · Figure 4c
linear pH response range	pH 4 to 8	—	—	Text Exact Reported	4 · 2.2 Electrical Characterization and pH Sensing · Figure 4c
pH cycling reversibility	no noticeable threshold-voltage shift when re-measured at pH 7 after pH 4 and pH 11 exposure	—	—	Text Qualitative	4 · 2.2 Electrical Characterization and pH Sensing · Figure 4d
pH sensitivityMarked as a best value within this paper	23 mV decade-1 (pH)	—	—	Text Rounded Reported	4 · 2.2 Electrical Characterization and pH Sensing · Figure 4c

scrambled-aptamer EG-FET cortisol control

Field-effect transistor measurements

scrambled-aptamer-functionalised CuHITP EG-FET control sensor · Electrode

Sensor functionalised with scrambled aptamer of similar length and GC content exposed to cortisol; compared against specific cortisol aptamer sensor.

Atmosphere: 0.01X PBS electrolyte
Geometry: scrambled-aptamer CuHITP extended gate connected to commercial p-channel MOSFET
Context: Scrambled aptamer control on conductive-MOF heterostructure
Measurement source: 8 · 2.3.2 Cortisol Sensing · Figure 6c; Figure S11

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
scrambled control response at 1 nM cortisol	roughly 20% of specific aptamer sensor	0.2 fraction of specific aptamer response	roughly	Text Approximate	8 · 2.3.2 Cortisol Sensing · Figure S11c cited